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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (N-Term)

Details for Product anti-GAPDH Antibody No. ABIN1002341, Supplier: Log in to see
Antigen
  • BEST:GH12586
  • cb609
  • CG8893
  • CG12055
  • Dmel\\CG8893
  • Dmel\\CG12055
  • g3pd
  • G3PD
  • G3PDH
  • GA3PDH
  • GADPH
  • gadph
  • GAP
  • GAPC-2
  • gapd
  • GAPD
  • Gapd
  • gapdh
  • GAPDH
  • Gapdh
  • GAPDH-1
  • Gapdh-1
  • gapdh-1
  • Gapdh-2
  • GAPDH1
  • GAPDH2
  • Gapdh13F
  • Gapdh43E
  • GAPDHI
  • GAPDH I
  • GAPDH II
  • GB14798
  • gh12586
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • KNC-NDS6
  • mg:bb02e05
  • T6J4.17
  • T6J4_17
  • wu:fb33a10
  • wu:ft80f05
Epitope
N-Term
98
47
21
11
10
10
9
8
8
7
6
6
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
541
218
188
107
58
51
44
33
29
24
18
18
17
13
12
11
8
8
7
7
6
6
6
6
6
6
5
5
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
332
227
57
31
20
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
41
37
18
6
6
6
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
585
275
149
127
85
58
44
26
22
14
10
9
7
6
5
5
4
4
2
2
2
2
1
1
1
1
Supplier
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Immunogen Rabbit polyclol GAPDH antibody was raised against a 16 amino acid peptide from near the amino-terminus of human GAPDH (Genbank accession No. P04406).
Blocking Peptide Blocking peptide for this product available: ABIN1004139
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name GAPDH / G3PDH / GAPD (GAPDH Antibody Abstract)
Background Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Application Notes GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Sirover: "New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells." in: Journal of cellular biochemistry, Vol. 95, Issue 1, pp. 45-52, 2005

Chuang, Hough, Senatorov: "Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases." in: Annual review of pharmacology and toxicology, Vol. 45, pp. 269-90, 2005