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Glucocorticoid Induced TNF Receptor Ligand antibody

Details for Product No. ABIN1002355, Supplier: Log in to see
Human, Mouse (Murine)
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol GITRL antibody was raised against purified recombint human GITR ligand (Genbank accession Q9UNG2).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappaB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-kappaB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappaB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
Application Notes GITRL antibody can be used for the detection of GITRL by Western blot at 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Gaur, Aggarwal: "Regulation of proliferation, survival and apoptosis by members of the TNF superfamily." in: Biochemical pharmacology, Vol. 66, Issue 8, pp. 1403-8, 2003

Ronchetti, Nocentini, Riccardi, Pandolfi: "Role of GITR in activation response of T lymphocytes." in: Blood, Vol. 100, Issue 1, pp. 350-2, 2002

Gurney, Marsters, Huang, Pitti, Mark, Baldwin, Gray, Dowd, Brush, Heldens, Schow, Goddard, Wood, Baker, Godowski, Ashkenazi: "Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR." in: Current biology : CB, Vol. 9, Issue 4, pp. 215-8, 1999

Background publications Shimizu, Yamazaki, Takahashi, Ishida, Sakaguchi: "Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance." in: Nature immunology, Vol. 3, Issue 2, pp. 135-42, 2002