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HRK antibody (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain))

Details for Product anti-HRK Antibody No. ABIN1002403, Supplier: Log in to see
Antigen
  • AI838259
  • Bid3
  • DP5
  • Dp5
  • HARAKIRI
  • harakiri
Reactivity
Human, Mouse (Murine)
45
15
7
Host
Rabbit
45
Clonality
Polyclonal
Conjugate
This HRK antibody is un-conjugated
3
3
2
2
2
2
Application
ELISA, Western Blotting (WB)
25
21
16
4
1
Supplier
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Immunogen HRK antibody was raised against a 15 amino acid peptide from near the center of human Hrk.
Blocking Peptide Blocking peptide for this product available: ABIN2861680
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Harakiri (Neuronal Death Protein DP5) (HRK Antibody Abstract)
Background Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
Application Notes This antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 myg/ml. Antibody can also be used for ELISA and might be suited for other applications not tested so far. This antibody is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Lockshin, Osborne, Zakeri: "Cell death in the third millennium." in: Cell death and differentiation, Vol. 7, Issue 1, pp. 2-7, 2000

Background publications Imaizumi, Tsuda, Imai, Wanaka, Takagi, Tohyama: "Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death." in: The Journal of biological chemistry, Vol. 272, Issue 30, pp. 18842-8, 1997

Inohara, Ding, Chen, Núñez: "harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)." in: The EMBO journal, Vol. 16, Issue 7, pp. 1686-94, 1997