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CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase)

Details for Product anti-CHUK Antibody No. ABIN1002503, Supplier: Log in to see
Antigen
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Alternatives
anti-Human CHUK antibody for Western Blotting
Reactivity
Human
357
161
110
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
277
83
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
321
153
95
70
61
49
43
29
26
19
9
5
3
3
2
2
1
1
1
Options
Supplier
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Immunogen Rabbit polyclol IKKalpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK( , which differs from corresponding murine sequence by four amino acids.
Blocking Peptide Blocking peptide for this product available: ABIN1003711
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name IkB Kinase alpha (CHUK Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IKappaB degradation and NF-kappaB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKa and IKKbeta interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkB-alpha. IKKalpha is expressed in variety of human tissues.
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response
Application Notes This antibody is human-specific and can be used for detection of IKK-alpha by Western blot at 1:1000 to 1:2000 dilution. (Optimal dilution should be determined by user.) An 85 kDa band should be detected. antibody has no cross response to IKK-beta or IKK-gamma. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Régnier, Song, Gao, Goeddel, Cao, Rothe: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Woronicz, Gao, Cao, Rothe, Goeddel: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).

Product cited in: Zandi, Rothwarf, Delhase, Hayakawa, Karin: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).