anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase)

Details for Product anti-CHUK Antibody No. ABIN1002503
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Synonyms ikk, ikk1, ikka, ikkalpha, CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, AI256658, Chuk1, Fbx24, Fbxo24, Ikka, zgc:56539
(339), (110), (82), (2), (2), (1)
(287), (55), (10), (2)
This CHUK antibody is un-conjugated
(14), (10), (10), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2)
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(311), (140), (102), (45), (41), (40), (33), (20), (10), (9), (9), (3), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 15 to 19 Business Days
Immunogen Rabbit polyclol IKKalpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK( , which differs from corresponding murine sequence by four amino acids.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name IkB Kinase alpha (CHUK Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IKappaB degradation and NF-kappaB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKa and IKKbeta interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkB-alpha. IKKalpha is expressed in variety of human tissues.
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway
Application Notes This antibody is human-specific and can be used for detection of IKK-alpha by Western blot at 1:1000 to 1:2000 dilution. (Optimal dilution should be determined by user.) An 85 kDa band should be detected. antibody has no cross response to IKK-beta or IKK-gamma. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Zandi, Rothwarf, Delhase et al.: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).

Background publications Woronicz, Gao, Cao et al.: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Catalog No. ABIN1002503
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