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IKBKG antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma) (N-Term)

Details for Product anti-IKBKG Antibody No. ABIN1002508, Supplier: Log in to see
Antigen
  • 1110037D23Rik
  • AI848108
  • AI851264
  • AMCBX1
  • AW124339
  • CG16910
  • dIKK
  • dIKK-gamma
  • Dmel\\CG16910
  • DmIKK-gamma
  • dmIKKgamma
  • Dmikkgamma
  • DmIKKgamma
  • FIP-3
  • FIP3
  • Fip3p
  • fj33c07
  • IKBKG
  • IKBKG_1
  • IKBKG_2
  • IKK
  • IKK-gamma
  • IKKg
  • IKKgamma
  • IKK[g]
  • IKK[[gamma]]
  • IP
  • IP1
  • IP2
  • IPD2
  • Kenny
  • Key
  • KEY
  • MGC97885
  • nemo
  • Nemo
  • NEMO
  • wu:fj33c07
  • ZC2HC9
  • zgc:113492
Alternatives
anti-Human IKBKG antibody for Western Blotting
Epitope
N-Term
31
28
20
13
11
11
9
9
8
7
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3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
302
96
65
5
4
4
3
2
2
2
2
2
1
1
Host
Rabbit
215
94
2
2
1
Clonality
Polyclonal
Conjugate
This IKBKG antibody is un-conjugated
10
9
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4
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3
2
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Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
239
105
74
45
44
39
24
23
5
4
3
2
1
1
Supplier
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Immunogen Rabbit polyclol IKK( antibody was raised against a peptide (CQYQAPDMDTLQIHVME) corresponding to amino acids 400 to 416 of human IKK( , which are identical to those of mouse homologue .
Blocking Peptide Blocking peptide for this product available: ABIN1004146
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name IkB Kinase gamma (IKBKG Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The IkB kinase (IKKalpha and IKKbeta) phosphorylates IkB and mediates NF-kappaB activation. A novel molecule in the IKK complex was recently identified and termed IKKgamma/NEMO/FIP3/IKKAP1. IKKgamma interacts with IKKbeta and is required for the activation of IKK complex and NF-kappaB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kappaB activation.
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes IKK-gamma antibody can be used for detection of IKK-gamma by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) A 52 kDa band should be detected. IKK-gamma has no cross response to IKK-alpha or IKK-beta. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Li, Kang, Friedman, Tarassishin, Ye, Kovalenko, Wallach, Horwitz: "Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 3, pp. 1042-7, 1999 (PubMed).

Mercurio, Murray, Shevchenko, Bennett, Young, Li, Pascual, Motiwala, Zhu, Mann, Manning: "IkappaB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex." in: Molecular and cellular biology, Vol. 19, Issue 2, pp. 1526-38, 1999 (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).

Background publications Yamaoka, Courtois, Bessia, Whiteside, Weil, Agou, Kirk, Kay, Israël: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).