Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase Complex-Associated Protein (IKBKAP) antibody

Details for Product No. ABIN1002511
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Antigen
Synonyms IKBKAP, DKFZp469N1415, ABA-OVERLY SENSITIVE 1, ELO2, ELONGATA 2, MSH12.15, MSH12_15, 3110040G09Rik, 6030413P05, C78473, Elp1, IKAP, DYS, ELP1, FD, IKI3, TOT1
Reactivity
Human, Mouse (Murine)
(59), (23), (14), (1), (1)
Host
Rabbit
(52), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(52), (26), (11), (10), (10), (7), (4), (3)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1002511
514.01 $
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Immunogen IKAP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IKAP.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name IKK Complex Associated Protein (IKBKAP)
Background IKAP was initially identified as a scaffold protein of the IkappaB kinase complex that could bind to IKKalpha, IKKbeta, NF-kappaB, and the NF-kappaB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.
Application Notes This antibody can be used for detection of IKAP by Western blot at 0.5 to 1 myg/ml. This antibody is human, mouse reactive. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Cohen, Henzel, Baeuerle: "IKAP is a scaffold protein of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 292-6, 1998 (PubMed).

Krappmann, Hatada, Tegethoff et al.: "The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component." in: The Journal of biological chemistry, Vol. 275, Issue 38, pp. 29779-87, 2000 (PubMed).

Anderson, Coli, Daly et al.: "Familial dysautonomia is caused by mutations of the IKAP gene." in: American journal of human genetics, Vol. 68, Issue 3, pp. 753-8, 2001 (PubMed).

Hawkes, Otero, Winkler et al.: "Purification and characterization of the human elongator complex." in: The Journal of biological chemistry, Vol. 277, Issue 4, pp. 3047-52, 2002 (PubMed).

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