IL-1R Associated Kinase (C-Term) antibody

Details for Product No. ABIN1002515
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Antigen
Epitope
C-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1002515
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Immunogen Rabbit polyclol IRAK antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK (GenBank accession no. P51617).
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Application Notes IRAK antibody can be used for Western blot at 1 µg/ml and for immunoprecipitation with 2 to 4 µg per sample. (Optimal dilution should be determined by user.) THP-1 or HeLa whole cell lysate can be used as positive control and an 80 kDa band should be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. IRAK antibody is human, mouse, and rat reactive, and has no cross reactivity to IRAK2.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).

Trofimova, Sprenkle, Green et al.: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996 (PubMed).

Robinson, Shibuya, Mui et al.: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997 (PubMed).

Huang, Gao, Li et al.: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998 (PubMed).

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