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Details for Product No. ABIN1002518

IL-1R Associated Kinase 2 (C-Term) antibody

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Antigen
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1002518
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days
Immunogen Rabbit polyclol mIRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy-terminus of mouse IRAK2a (GenBank accession no. AAO24761) which is common to all four isoforms. mIRAK2 antibody will also recognize human IRAK2.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappaB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
Application Notes IRAK2 antibody can be used for detection of mouse IRAK2 by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) A 65 kDa band should be detected. Anti-IRAK2 has no cross response to IRAK. Anti-IRAK2 will also recognize human IRAK2. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Muzio, Ni, Feng et al.: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997 (PubMed).

Zhang, Kirschning, Mancinelli et al.: "Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7611-4, 1999 (PubMed).

Yang, Mark, Gurney et al.: "Signaling events induced by lipopolysaccharide-activated toll-like receptor 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 639-43, 1999 (PubMed).

Hardy, ONeill: "The murine IRAK2 gene encodes four alternatively spliced isoforms, two of which are inhibitory." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27699-708, 2004 (PubMed).

Alternatives for antigen "IL-1R Associated Kinase 2", type "Antibodies"
Hosts (3)
Reactivities (2), (1)
Applications (3), (3), (1), (1)
Epitopes (1)
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