Interleukin 33 (IL33) (N-Term) antibody

Details for Product No. ABIN1002523
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Antigen
Synonyms IL33, C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, RP11-575C20.2, 9230117N10Rik, Il-33, Il1f11, RGD1311155
Epitope
N-Term
(33), (15), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(147), (48), (26), (4), (1)
Host
Rabbit
(112), (55), (5), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(15), (8), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
ELISA, Western Blotting (WB), Fluorescence Microscopy (FM)
(131), (118), (25), (24), (20), (14), (12), (9), (9), (6), (4), (4), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1002523
514.01 $
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Immunogen IL-33 antibody was raised in rabbits against a 19 amino acid peptide from near the amino terminus of human IL-33.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name IL-33
Background Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1a/b, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.
Application Notes IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunofluorescence microscopy and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Dinarello: "Interleukin-18, a proinflammatory cytokine." in: European cytokine network, Vol. 11, Issue 3, pp. 483-6, 2000 (PubMed).

Brint, Xu, Liu et al.: "ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance." in: Nature immunology, Vol. 5, Issue 4, pp. 373-9, 2004 (PubMed).

Schmitz, Owyang, Oldham et al.: "IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines." in: Immunity, Vol. 23, Issue 5, pp. 479-90, 2005 (PubMed).

Chackerian, Oldham, Murphy et al.: "IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2551-5, 2007 (PubMed).

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