You are viewing an incomplete version of our website. Please click to reload the website as full version.

Interferon Regulatory Factor 3 (IRF3) (Internal Region) antibody

Details for Product No. ABIN1002559, Supplier: Log in to see
Antigen
  • C920001K05Rik
  • IRF-3
  • IRF3
  • irf3
Alternatives
anti-Human Interferon Regulatory Factor 3 antibody for Western Blotting
Epitope
Internal Region
29
25
21
18
18
17
14
13
13
8
7
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
267
86
43
6
5
4
2
Host
Rabbit
212
80
2
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
247
113
71
65
44
38
36
23
15
11
9
4
3
3
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Rabbit polyclol IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3 (GenBank accession no. AAO24761). IRF3 antibody is human specific.
Blocking Peptide Blocking peptide for this product available: ABIN1004029
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Interferon Regulatory Factor 3 (IRF3 Antibody Abstract)
Background Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
Pathways TLR Signaling
Application Notes IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Malmgaard: "Induction and regulation of IFNs during viral infections." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 24, Issue 8, pp. 439-54, 2004 (PubMed).

Fitzgerald, McWhirter, Faia, Rowe, Latz, Golenbock, Coyle, Liao, Maniatis: "IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway." in: Nature immunology, Vol. 4, Issue 5, pp. 491-6, 2003 (PubMed).

Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, 2003 (PubMed).

Au, Moore, Lowther, Juang, Pitha: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).