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Interferon Regulatory Factor 3 (IRF3) (Internal Region) antibody

Details for Product No. ABIN1002559, Supplier: Log in to see
Antigen
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
Alternatives
anti-Human Interferon Regulatory Factor 3 antibody for Western Blotting
Epitope
Internal Region
28
21
19
18
18
14
13
13
11
7
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
245
85
44
6
5
4
2
Host
Rabbit
210
59
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
229
97
62
54
37
33
26
24
11
9
9
4
3
3
2
1
1
1
1
1
Supplier
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Immunogen Rabbit polyclol IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3 (GenBank accession no. AAO24761). IRF3 antibody is human specific.
Blocking Peptide Blocking peptide for this product available: ABIN1004029
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Interferon Regulatory Factor 3 (IRF3 Antibody Abstract)
Background Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
Pathways TLR Signaling
Application Notes IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Malmgaard: "Induction and regulation of IFNs during viral infections." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 24, Issue 8, pp. 439-54, 2004 (PubMed).

Sharma, tenOever, Grandvaux et al.: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, 2003 (PubMed).

Fitzgerald, McWhirter, Faia et al.: "IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway." in: Nature immunology, Vol. 4, Issue 5, pp. 491-6, 2003 (PubMed).

Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).