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Interleukin 32 (IL32) (Internal Region) antibody

Details for Product No. ABIN1002638, Supplier: Login to see
Antigen
  • IL-32alpha
  • IL-32beta
  • IL-32delta
  • IL-32gamma
  • NK4
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd
Epitope
Internal Region
30
14
13
7
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
127
22
3
Host
Rabbit
110
16
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
4
3
3
3
1
Application
ELISA, Western Blotting (WB)
108
61
42
13
5
5
5
3
2
1
1
1
Supplier
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Immunogen IL-32 antibody was raised against a 16 amino acid peptide from near the center of human IL-32 (Genbank accession No. AAH09401).
Blocking Peptide Blocking peptide for this product available: ABIN2861697
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Interleukin 32 (IL32 Antibody Abstract)
Background Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
Pathways
Application Notes IL-32 antibody can be used for the detection of IL-32 by Western blot at 2.5 – 5 µg/ml. (Optimal dilution should be determined by user.) This antibody detects the largest isoform of IL-32. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Cagnard, Letourneur, Essabbani et al.: "Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes." in: European cytokine network, Vol. 16, Issue 4, pp. 289-92, 2006 (PubMed).

Kim, Han, Azam et al.: "Interleukin-32: a cytokine and inducer of TNFalpha." in: Immunity, Vol. 22, Issue 1, pp. 131-42, 2005 (PubMed).

Dahl, Schall, He et al.: "Identification of a novel gene expressed in activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 2, pp. 597-603, 1992 (PubMed).