IRS-1 (Scaffolding Protein Important For Insulin Receptor Signaling) antibody

Details for Product No. ABIN1002694
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Antigen
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1002694
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen Rabbit polyclol IRS-1 antibody was raised against a 16 amino acid peptide from near the center of human IRS-1 (Genbank accession No. P35568).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.
Application Notes IRS-1antibody can be used for the detection of IRS-1 by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Giovannone, Scaldaferri, Federici et al.: "Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential." in: Diabetes/metabolism research and reviews, Vol. 16, Issue 6, pp. 434-41, 2001 (PubMed).

Ozes, Akca, Mayo et al.: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Waters, Pessin: "Insulin receptor substrate 1 and 2 (IRS1 and IRS2): what a tangled web we weave." in: Trends in cell biology, Vol. 6, Issue 1, pp. 1-4, 2004 (PubMed).

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