Livin (Novel Member in IAP Family) antibody

Details for Product No. ABIN1002744
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Quantity 100 μg
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Catalog No. ABIN1002744
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Immunogen Rabbit polyclol Livin antibody was raised with a synthetic peptide (CRAPVRSRVRTFLS) corresponding to amino acids 264 to 280 of the short form and 281 to 298 of the long form of human Livin . This sequence is identical between a and b forms of the Livin proteins.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-a and Livin-b. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.
Application Notes Livin antibody can be used for detection of Livin by Western blot at 2 to 4 µg/ml. (Optimal dilution should be determined by user.) Human Raji cell lysate can be used as a positive control and a band at 33 kDa can be detected. A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kasof, Gomes: "Livin, a novel inhibitor of apoptosis protein family member." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3238-46, 2001 (PubMed).

Lin, Deng, Huang et al.: "KIAP, a novel member of the inhibitor of apoptosis protein family." in: Biochemical and biophysical research communications, Vol. 279, Issue 3, pp. 820-31, 2001 (PubMed).

Ashhab, Alian, Polliack et al.: "Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern." in: FEBS letters, Vol. 495, Issue 1-2, pp. 56-60, 2001 (PubMed).

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