Lysine (K)-Specific Demethylase 1A (KDM1A) (N-Term) antibody

Details for Product No. ABIN1002761, Supplier: Log in to see
Antigen
  • KDM1
  • AOF2
  • KDM1A
  • DKFZp459N2450
  • aof2
  • kdm1
  • lsd1
  • xLSD1
  • BHC110
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • RGD1562975
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • ARABIDOPSIS LYSINE-SPECIFIC HISTONE DEMETHYLASE
  • ATLSD1
  • ATSWP1
  • F23N19.21
  • F23N19_21
  • LSD1-like 1
  • LYSINE-SPECIFIC HISTONE DEMETHYLASE
  • SWP1
  • amine oxidase (flavin containing) domain 2
  • lysine (K)-specific demethylase 1
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • Lysine-specific histone demethylase 1-1
  • AOF2
  • KDM1
  • KDM1A
  • kdm1a
  • Kdm1a
  • LDL1
Alternatives
anti-Human Lysine (K)-Specific Demethylase 1A antibody for Immunofluorescence (fixed cells)
Epitope
N-Term
18
9
9
8
8
7
7
6
5
5
5
5
4
3
3
3
3
2
2
2
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
210
122
95
24
24
11
10
7
5
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
161
50
3
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
177
61
61
48
31
26
16
14
10
8
6
6
3
3
1
1
1
1
Options
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Immunogen LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1.
Blocking Peptide Blocking peptide for this product available: ABIN2861774
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Lysine Specific Histone Demethylase 1 (KDM1A Antibody Abstract)
Background Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling
Application Notes LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Shi, Matson, Lan, Iwase, Baba, Shi: "Regulation of LSD1 histone demethylase activity by its associated factors." in: Molecular cell, Vol. 19, Issue 6, pp. 857-64, 2005 (PubMed).

Shi, Lan, Matson, Mulligan, Whetstine, Cole, Casero, Shi: "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." in: Cell, Vol. 119, Issue 7, pp. 941-53, 2004 (PubMed).

Kouzarides: "Histone methylation in transcriptional control." in: Current opinion in genetics & development, Vol. 12, Issue 2, pp. 198-209, 2002 (PubMed).

Nakamura, Mori, Tada, Krajewski, Rozovskaia, Wassell, Dubois, Mazo, Croce, Canaani: "ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation." in: Molecular cell, Vol. 10, Issue 5, pp. 1119-28, 2002 (PubMed).