Lysosome Associated Membrane Glycoprotein 2 (Autophagy Related Protein) antibody

Details for Product No. ABIN1002764
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Antigen
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1002764
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen Rabbit polyclol LAMP-2 antibody was raised against a 17 amino acid peptide from near the center of human LAMP-2 (Genbank accession No. NP_034815).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon’s disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.
Application Notes LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). HepG2 cell lysate can be used as positive control. Despite its predicted size, LAMP-2 migrates at 115kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. LAMP-2 antibody is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Granger, Green, Gabel et al.: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).

Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

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