MAPKAP1 antibody (Mitogen-Activated Protein Kinase Associated Protein 1) (Internal Region)

Details for Product anti-MAPKAP1 Antibody No. ABIN1002803, Supplier: Log in to see
Antigen
  • CG10105
  • Dmel\\CG10105
  • dSin1
  • sin1
  • MAPKAP1
  • im:7143088
  • si:ch73-242f23.1
  • mip1
  • sin1b
  • sin1g
  • MGC53034
  • JC310
  • MIP1
  • SIN1
  • SIN1b
  • SIN1g
  • AI591529
  • D230039K05Rik
  • Sin1
  • SAPK-interacting protein 1
  • mitogen-activated protein kinase associated protein 1
  • Sin1
  • MAPKAP1
  • mapkap1
  • Mapkap1
Alternatives
anti-Human MAPKAP1 antibody for Western Blotting
Epitope
Internal Region
17
14
8
4
4
3
3
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
82
44
38
12
10
5
3
3
3
2
1
1
1
Host
Rabbit
84
Clonality
Polyclonal
Conjugate
This MAPKAP1 antibody is un-conjugated
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
74
32
32
18
14
5
4
2
2
1
1
Options
Supplier
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Immunogen MAPKAP1 antibody was raised in rabbits against an 18 amino acid peptide from near the center of human MAPKAP1.
Blocking Peptide Blocking peptide for this product available: ABIN1003959
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name MAPKAP1 (MAPKAP1 Antibody Abstract)
Background MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
Pathways PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events
Application Notes MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Jacinto, Facchinetti, Liu, Soto, Wei, Jung, Huang, Qin, Su: "SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity." in: Cell, Vol. 127, Issue 1, pp. 125-37, 2006 (PubMed).

Wullschleger, Loewith, Hall: "TOR signaling in growth and metabolism." in: Cell, Vol. 124, Issue 3, pp. 471-84, 2006 (PubMed).

Cheng, Zhang, Kim, Zhao, Zhao, Su: "Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 5955-64, 2005 (PubMed).

Schroder, Cloonan, Bushell, Sculley: "Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene." in: Gene, Vol. 339, pp. 17-23, 2004 (PubMed).

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