Mcl-1 (Bcl-2 Protein Family Member) (C-Term) antibody

Details for Product No. ABIN1002829
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Antigen
Epitope
C-Term
(1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(2)
Host
Rabbit
(2)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(2), (2), (2)
Pubmed 3 references available
Catalog no. ABIN1002829
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human Mcl-1 (GenBank accession no. NP_068779).
Isotype IgG
Purification Ion exchange chromatography purified.
Background Myeloid cell leukemia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.
Application Notes Mcl-1 antibody can be used for detection of the large isoform of Mcl-1 only by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kozopas, Yang, Buchan et al.: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993 (PubMed).

Cuconati, Mukherjee, Perez et al.: "DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells." in: Genes & development, Vol. 17, Issue 23, pp. 2922-32, 2003 (PubMed).

Edwards, Derouet, Howse et al.: "Regulation of neutrophil apoptosis by Mcl-1." in: Biochemical Society transactions, Vol. 32, Issue Pt3, pp. 489-92, 2004 (PubMed).

Hosts (2)
Reactivities (2)
Applications (2), (2), (2)
Epitopes (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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