Interferon Induced with Helicase C Domain 1 (IFIH1) (Internal Region) antibody

Details for Product No. ABIN1002836
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Antigen
Synonyms MDA5, Hlcd, IDDM19, MDA-5, RLR-2, 9130009C22Rik, Helicard, RGD1560496
Epitope
Internal Region
(14), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(65), (33), (1), (1), (1)
Host
Rabbit
(56), (9), (5)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(60), (39), (25), (18), (5), (4), (3), (3), (3), (2), (1)
Pubmed 4 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1002836
514.01 $
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Immunogen MDA5 antibody was raised in rabbits against a 16 amino acid peptide from near the center of human MDA5.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name MDA5
Background The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Application Notes MDA5 antibody can be used for detection of MDA5 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Kang, Gopalkrishnan, Lin et al.: "Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene." in: Oncogene, Vol. 23, Issue 9, pp. 1789-800, 2004 (PubMed).

Andrejeva, Childs, Young et al.: "The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17264-9, 2004 (PubMed).

Akira, Uematsu, Takeuchi: "Pathogen recognition and innate immunity." in: Cell, Vol. 124, Issue 4, pp. 783-801, 2006 (PubMed).

Hiscott, Nguyen, Arguello et al.: "Manipulation of the nuclear factor-kappaB pathway and the innate immune response by viruses." in: Oncogene, Vol. 25, Issue 51, pp. 6844-67, 2006 (PubMed).

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