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MICA antibody (MHC Class I Polypeptide-Related Sequence A)

Details for Product anti-MICA Antibody No. ABIN1002843, Supplier: Log in to see
Antigen
  • FLJ60820
  • Ica
  • MGC111087
  • MIC-A
  • mICA
  • PERB11.1
Reactivity
Human, Mouse (Murine)
189
33
15
14
12
8
7
Host
Rabbit
119
67
1
Clonality
Polyclonal
Conjugate
This MICA antibody is un-conjugated
18
7
7
7
6
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
123
100
62
56
29
22
17
12
12
9
5
3
1
1
1
Supplier
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Immunogen MICA antibody was raised in rabbits against a 16 amino acid peptide from near the carboxy terminus of human MICA.
Blocking Peptide Blocking peptide for this product available: ABIN1004396
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name MICA (MICA Antibody Abstract)
Background Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
Research Area Immunology, Innate Immunity, Adaptive Immunity
Application Notes MICA antibody can be used for detection of MICA by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Rudolph, Stanfield, Wilson: "How TCRs bind MHCs, peptides, and coreceptors." in: Annual review of immunology, Vol. 24, pp. 419-66, 2006 (PubMed).

Maccalli, Pende, Castelli, Mingari, Robbins, Parmiani: "NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity." in: European journal of immunology, Vol. 33, Issue 7, pp. 2033-43, 2003 (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Bahram, Bresnahan, Geraghty, Spies: "A second lineage of mammalian major histocompatibility complex class I genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 14, pp. 6259-63, 1994 (PubMed).