NALP1 (Caspase Interacting Protein) antibody

Details for Product No. ABIN1002875
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1002875
514.01 $
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Immunogen Rabbit polyclol LP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy-terminus of human LP1 (GenBank accession no. AAG30288).
Isotype IgG
Purification Immunoaffinity chromatography purified IgG.
Background NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.
Application Notes NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) U937 cell lysate can be used as a positive control. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. NALP1 antibody is human specific.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hlaing, Guo, Dilley et al.: "Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the mammalian Ced-4 family of apoptosis proteins." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9230-8, 2001 (PubMed).

Chu, Pio, Xie et al.: "A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9239-45, 2001 (PubMed).

Tschopp, Martinon, Burns: "NALPs: a novel protein family involved in inflammation." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 2, pp. 95-104, 2003 (PubMed).

Sanz, Calasanz, Andreu et al.: "NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukaemia cells." in: The Biochemical journal, Vol. 384, Issue Pt 2, pp. 281-6, 2004 (PubMed).

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