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TANK-Binding Kinase 1 (TBK1) (C-Term) antibody

Details for Product No. ABIN1002907, Supplier: Log in to see
Antigen
  • nak
  • t2k
  • TBK1
  • wu:fk70c05
  • zgc:136548
  • NAK
  • T2K
  • 1200008B05Rik
  • AI462036
  • AW048562
Alternatives
anti-Human TANK-Binding Kinase 1 antibody for Western Blotting
Epitope
C-Term
28
23
16
15
6
6
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
107
56
25
4
4
4
4
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
98
19
10
Clonality
Polyclonal
Conjugate
Un-conjugated
9
7
7
7
5
5
2
2
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
108
59
30
15
8
6
3
3
2
2
2
1
Supplier
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Immunogen Rabbit polyclol K antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human K/TBK1.
Blocking Peptide Blocking peptide for this product available: ABIN1003826
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name NF-kB Activating Kinase (TBK1 Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. Phosphorylation of I-kappaB by IkappaB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-kappaB. The IKK complex contains IKKalpha, IKKbeta, and IKKgamma. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kappaB-activating kinase), and T2K. NAK/TBK1 activates IKKbeta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kappaB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
Pathways TLR Signaling
Application Notes NAK antibody can be used for detection of NAK/TBK1 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) An 84 kDa band should be detected. It is human, mouse and rat reactive and has no cross response to IKK-alpha, IKK-beta, IKK-gamma, or IKK-epsilon. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Tojima, Fujimoto, Delhase et al.: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000 (PubMed).

Background publications Pomerantz, Baltimore: "NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase." in: The EMBO journal, Vol. 18, Issue 23, pp. 6694-704, 2000 (PubMed).