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Nucleotide-Binding Oligomerization Domain Protein 2 (New CARD Containing Protein) (N-Term) antibody

Details for Product No. ABIN1002933, Supplier: Log in to see
Antigen
Epitope
N-Term
1
Reactivity
Human
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1
1
1
Supplier
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Immunogen Rabbit NOD2 polyclol antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2 (GenBank accession no. Q9HC29).
Blocking Peptide Blocking peptide for this product available: ABIN1004165
Isotype IgG
Purification Immunoaffinity chromatography purified IgG
Background Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-kappaB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease that is a main type of chronic inflammatory bowel disease.
Application Notes NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 µg/ml. (Optimal dilution should be determined by user.) NOD2 has no cross-reaction with NOD1. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Ogura, Inohara, Benito, Chen, Yamaoka, Nunez: "Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4812-8, 2001

Hugot, Chamaillard, Zouali, Lesage, Cézard, Belaiche, Almer, Tysk, OMorain, Gassull, Binder, Finkel, Cortot, Modigliani, Laurent-Puig, Gower-Rousseau, Macry, Colombel, Sahbatou, Thomas: "Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 599-603, 2001

Ogura, Bonen, Inohara, Nicolae, Chen, Ramos, Britton, Moran, Karaliuskas, Duerr, Achkar, Brant, Bayless, Kirschner, Hanauer, Nuñez, Cho: "A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 603-6, 2001

Inohara, Koseki, del Peso, Hu, Yee, Chen, Carrio, Merino, Liu, Ni, Núñez: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999