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Nudel (Protein Involved in Neuronal Development) antibody

Details for Product No. ABIN1002935, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel (GenBank accession no. AAF97497). Nudel antibody is human and mouse reactive.
Blocking Peptide Blocking peptide for this product available: ABIN1004414
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration, phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.
Application Notes Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Liang, Yu, Li et al.: "Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein." in: The Journal of cell biology, Vol. 164, Issue 4, pp. 557-66, 2004 (PubMed).

Gupta, Tsai: "Cyclin-dependent kinase 5 and neuronal migration in the neocortex." in: Neuro-Signals, Vol. 12, Issue 4-5, pp. 173-9, 2003 (PubMed).

Niethammer, Smith, Ayala et al.: "NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein." in: Neuron, Vol. 28, Issue 3, pp. 697-711, 2001 (PubMed).

Liu, Steward, Luo: "Drosophila Lis1 is required for neuroblast proliferation, dendritic elaboration and axonal transport." in: Nature cell biology, Vol. 2, Issue 11, pp. 776-83, 2001 (PubMed).