OMI (IAP Inhibitor) antibody

Details for Product No. ABIN1002939
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Antigen
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN1002939
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen Rabbit polyclol OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human OMI (GenBank accession no. AAB94569).
Isotype IgG
Purification Ion exchange chromatography purified.
Background Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.
Application Notes OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Crook, Clem, Miller: "An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif." in: Journal of virology, Vol. 67, Issue 4, pp. 2168-74, 1993 (PubMed).

Suzuki, Imai, Nakayama et al.: "A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death." in: Molecular cell, Vol. 8, Issue 3, pp. 613-21, 2001 (PubMed).

Vaux, Silke: "Mammalian mitochondrial IAP binding proteins." in: Biochemical and biophysical research communications, Vol. 304, Issue 3, pp. 499-504, 2003 (PubMed).

Liston, Fong, Korneluk: "The inhibitors of apoptosis: there is more to life than Bcl2." in: Oncogene, Vol. 22, Issue 53, pp. 8568-80, 2003 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1)
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