ORAI1 antibody (ORAI Calcium Release-Activated Calcium Modulator 1) (C-Term)

Details for Product anti-ORAI1 Antibody No. ABIN1002944, Supplier: Log in to see
Antigen
  • im:7146264
  • orai1
  • tmem142a
  • zgc:109721
  • orai2
  • ORAI1
  • LOC100218031
  • CRACM1
  • ORAT1
  • TMEM142A
  • D730049H07Rik
  • Tmem142a
  • orai-1
  • RGD1311873
  • tmem142b
  • ORAI calcium release-activated calcium modulator 1b
  • ORAI calcium release-activated calcium modulator 1
  • ORAI calcium release-activated calcium modulator 2
  • orai1b
  • orai1
  • ORAI1
  • Orai1
  • LOC100218031
  • orai2
Alternatives
anti-Rat (Rattus) ORAI1 antibody for ELISA
Epitope
C-Term
42
11
11
10
10
9
9
8
3
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
120
51
27
7
5
5
4
4
4
3
3
1
1
1
Host
Rabbit
83
21
16
Clonality
Polyclonal
Conjugate
This ORAI1 antibody is un-conjugated
5
5
5
5
5
5
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
108
78
50
20
8
6
5
1
1
Options
Supplier
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Immunogen ORAI1 antibody was raised in rabbits against a 16 amino acid peptide from near the carboxy terminus of human ORAI1.
Blocking Peptide Blocking peptide for this product available: ABIN1003836
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ORAI1 (ORAI1 Antibody Abstract)
Background Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Pathways TCR Signaling
Application Notes ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Soboloff, Spassova, Dziadek, Gill: "Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication." in: Biochimica et biophysica acta, Vol. 1763, Issue 11, pp. 1161-8, 2006 (PubMed).

Background publications Feske, Gwack, Prakriya, Srikanth, Puppel, Tanasa, Hogan, Lewis, Daly, Rao: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).