ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibody

Details for Product No. ABIN1002944
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Antigen
Synonyms im:7146264, orai1, tmem142a, zgc:109721, orai2, ORAI1, LOC100218031, CRACM1, ORAT1, TMEM142A, D730049H07Rik, Tmem142a, orai-1, RGD1311873, tmem142b
Epitope
C-Term
(28), (14), (12), (8), (7), (3), (2), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(82), (40), (22), (2), (2), (2), (2)
Host
Rabbit
(57), (19), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(81), (50), (38), (13), (8), (6), (1)
Pubmed 2 references available
Quantity 100 μg
Shipping to United States (Change)
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Catalog No. ABIN1002944
514.01 $
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Immunogen ORAI1 antibody was raised in rabbits against a 16 amino acid peptide from near the carboxy terminus of human ORAI1.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ORAI1
Background Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Application Notes ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Soboloff, Spassova, Dziadek et al.: "Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication." in: Biochimica et biophysica acta, Vol. 1763, Issue 11, pp. 1161-8, 2006 (PubMed).

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