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ORAI3 antibody (ORAI Calcium Release-Activated Calcium Modulator 3) (N-Term)

Details for Product anti-ORAI3 Antibody No. ABIN1002948, Supplier: Log in to see
Antigen
  • ORAI3
  • TMEM142C
  • 9930124N15
  • BC061259
  • Tmem142c
  • RGD1306538
Epitope
N-Term
35
26
18
Reactivity
Human, Mouse (Murine), Rat (Rattus)
69
20
18
Host
Rabbit
55
14
Clonality
Polyclonal
Conjugate
This ORAI3 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
67
41
13
10
9
5
5
3
3
1
Supplier
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Immunogen ORAI3 antibody was raised in rabbits against a 15 amino acid peptide from near the amino terminus of human ORAI3.
Blocking Peptide Blocking peptide for this product available: ABIN1004170
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name ORAI3 (ORAI3 Antibody Abstract)
Background Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
Application Notes ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Soboloff, Spassova, Dziadek et al.: "Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication." in: Biochimica et biophysica acta, Vol. 1763, Issue 11, pp. 1161-8, 2006 (PubMed).

Background publications Mercer, Dehaven, Smyth et al.: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, 2006 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).