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P21-Activated Kinase 4 (PAK4) antibody

Details for Product No. ABIN1002957
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Antigen
Synonyms 5730488L07Rik, AW555722, mKIAA1142
Reactivity
Human
(108), (57), (48), (38), (37), (37), (13), (1)
Host
Rabbit
(95), (16), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(81), (44), (30), (28), (18), (8), (7), (2), (1)
Pubmed 3 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1002957
514.01 $
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Immunogen PAK4 antibody was raised against a 13 amino acid peptide from near the center of human PAK4.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name p21-Activated Kinase 4
Background The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.
Application Notes This antibody can be used for the detection of PAK4 by Western blot at 1 - 2 myg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. This antibody is human reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Abo, Qu, Cammarano et al.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia." in: The EMBO journal, Vol. 17, Issue 22, pp. 6527-40, 1999 (PubMed).

Jaffer, Chernoff: "p21-activated kinases: three more join the Pak." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 7, pp. 713-7, 2002 (PubMed).

Cotteret, Jaffer, Beeser et al.: "p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD." in: Molecular and cellular biology, Vol. 23, Issue 16, pp. 5526-39, 2003 (PubMed).

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