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P53-Regulated Apoptosis-Inducing Protein 1 (TP53AIP1) antibody

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Synonyms P53AIP1
(38), (2), (2)
(37), (1)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(16), (14), (12), (11), (8), (4), (4), (1), (1)
Pubmed 4 references available
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Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 15 to 19 Business Days
Immunogen Rabbit polyclol p53AIP antibody was raised against a 15 amino acid peptide from near the amino terminus of human p53AIP (GenBank accession no. BAB16421).
Blocking Peptide Blocking peptide for this product available: ABIN2861889
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name p53AIP1 (TP53AIP1 Antibody Abstract)
Background The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist, this antibody will detect all three.
Application Notes p53AIP antibody can be used for detection of p53AIP by Western blot at 4 – 8 myg/ml. (Optimal dilution should be determined by user.) HL60 cell lysate can be used as positive control. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. p53AIP antibody is human and mouse reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Yoshida, Monden, Nakamura et al.: "Adenovirus-mediated p53AIP1 gene transfer as a new strategy for treatment of p53-resistant tumors." in: Cancer science, Vol. 95, Issue 1, pp. 91-7, 2004 (PubMed).

Okamura, Arakawa, Tanaka et al.: "p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis." in: Molecular cell, Vol. 8, Issue 1, pp. 85-94, 2001 (PubMed).

Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

Background publications Kern, Pietenpol, Thiagalingam et al.: "Oncogenic forms of p53 inhibit p53-regulated gene expression." in: Science (New York, N.Y.), Vol. 256, Issue 5058, pp. 827-30, 1992 (PubMed).

Catalog No. ABIN1002964
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