PERP (Mediator of p53 Induced Apoptosis) antibody

Details for Product No. ABIN1002988
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN1002988
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen Rabbit polyclol PERP antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human PERP, which differ from the mouse sequence by three amino acids.
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Application Notes PERP antibody can be used for detection of PERP by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) A431 cell lysate can be used as positive control and a 21 kDa band can be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa et al.: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

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