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PID (Involved in p53 Deacetylation) antibody

Details for Product No. ABIN1002999, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol PID antibody was raised against a synthetic peptide (PAPSHPASTNEPIVLED) corresponding to amino acids 652 to 668 of human PID/MTA2 , which differ from the mouse sequence by one amino acid .
Blocking Peptide Blocking peptide for this product available: ABIN1004434
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 , p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.
Application Notes PID antibody can be used for detection of PID by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) HeLa cell lysate can be used as positive control and a 75 kDa band can be detected. It is human, mouse, and rat reactive and has no cross response to MTA1. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Tanaka, Arakawa, Yamaguchi, Shiraishi, Fukuda, Matsui, Takei, Nakamura: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa, Nemoto, Shibue, Yamashita, Tokino, Taniguchi, Tanaka: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Product cited in: Oda, Arakawa, Tanaka, Matsuda, Tanikawa, Mori, Nishimori, Tamai, Tokino, Nakamura, Taya: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).