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Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN1003029, Supplier: Log in to see
Antigen
  • PDPN
  • AGGRUS
  • GP36
  • GP40
  • Gp38
  • HT1A-1
  • OTS8
  • PA2.26
  • T1A
  • T1A-2
  • OTS-8
  • RANDAM-2
  • T1-alpha
  • T1a
  • T1alpha
  • E11
  • RTI40
Reactivity
Human
174
58
16
3
Host
Mouse
100
56
27
25
13
10
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Podoplanin antibody is un-conjugated
15
11
9
8
5
5
5
5
3
2
1
1
Application
Immunohistochemistry (IHC)
135
65
64
55
36
28
26
14
5
4
3
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen Membrane protein fraction of isolated human glomeruli
Clone 4D5aE5E6
Isotype IgG1 kappa
Specificity The antibody is human specific
Purification Protein G affinity chromatography
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Monoclonals were produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen. The mouse IgG1 antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
Pathways
Application Notes Immunohistochemistry: Cryo sections (acetone fixed) Paraffin sections (after autoclave or microwave treatment in 0.01 M citrate buffer, pH 6.0). Recommended working concentration is 0.5 µg/ml. Optimal dilutions should be determined by each laboratory for each application.
Restrictions For Research Use only
Format Liquid
Buffer PBS (pH 6.0) w/o preservative
Preservative Without preservative
Handling Advice Reconstituted antibody can also be aliquotted and stored at -20 °C to -70 °C for at least 6 months without detectable loss of activity.
Storage -20 °C
Product cited in: Kerjaschki, Huttary, Raab et al.: "Lymphatic endothelial progenitor cells contribute to de novo lymphangiogenesis in human renal transplants." in: Nature medicine, Vol. 12, Issue 2, pp. 230-4, 2006 (PubMed).

Kriehuber, Breiteneder-Geleff, Groeger et al.: "Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages." in: The Journal of experimental medicine, Vol. 194, Issue 6, pp. 797-808, 2001 (PubMed).

Breiteneder-Geleff, Soleiman, Kowalski et al.: "Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium." in: The American journal of pathology, Vol. 154, Issue 2, pp. 385-94, 1999 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman et al.: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).