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Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN1003032, Supplier: Login to see
Antigen
  • PDPN
  • AGGRUS
  • GP36
  • GP40
  • Gp38
  • HT1A-1
  • OTS8
  • PA2.26
  • T1A
  • T1A-2
  • OTS-8
  • RANDAM-2
  • T1-alpha
  • T1a
  • T1alpha
  • E11
  • RTI40
Reactivity
Human
181
59
16
3
Host
Rabbit
101
61
27
26
14
10
2
Clonality
Polyclonal
Conjugate
This Podoplanin antibody is un-conjugated
15
11
9
8
5
5
5
5
3
2
1
1
Application
Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)
141
70
67
55
38
28
27
14
5
4
3
3
3
3
2
2
2
1
1
1
1
Supplier
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Immunogen Recombint ectodomain of human gp36 (Podoplanin)
Isotype IgG
Specificity This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by immunostaining. Optimal dilutions should be determined by each laboratory for each application.
Purification Protein A affinity chromatography
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Produced from sera of rabbits immunized with the recombinant ectodomain of Podoplanin (gp36). Podoplanin is a highly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, Podoplanin colocalized with VEGFR-3/FLT-4, another marker for lymphatic endothelial cells.
Pathways
Application Notes Immunofluorescence: Use 1:750 to 1:1000 dilutions. Western Blotting: Use 1-5 µg/ml.
Restrictions For Research Use only
Format Liquid
Buffer PBS (pH 7.4) w/o preservative
Preservative Without preservative
Handling Advice Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 6 months without detectable loss of activity.
Storage -20 °C
Product cited in: Schacht, Ramirez, Hong et al.: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Zimmer, Oeffner, Von Messling et al.: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman et al.: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).