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Hepatitis B Surface Antigen (HBSAg) (AA 132-137) antibody
| Antigen | Hepatitis B Surface Antigen (HBSAg) |
| Binding Site |
 Alternatives AA 132-137 |
| Clonality | Monoclonal (3-00E-007) |
| Host |
Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
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4 references available |
| Catalog no. | ABIN100306 |
| Quantity | 6ml (1 to 4 ug/ml) (Variants) |
| Price | 203.50 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Will be delivered in 3 to 4 Business Days |
Additional Information
| Immunogen | BALB/c mice were immunized with purified HBsAg subtype AYW3. |
| Format | Supernatant (prediluted) |
| Isotype | IgG1 kappa |
| Clone | 3-00E-007 |
| Specificity | This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues. |
Application Details
| Application Notes | Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Infected liver. Cellular localization: Cytoplasmic |
| Concentration | 1 to 4 ug/ml |
| Purification | Supernatant (prediluted) |
| Buffer | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Virology |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
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Couroucue, Soulier: "The a(w) subdeterminants." in: Bibliotheca haematologica, Vol. 42, pp. 31-41, 1976 (PubMed).
Hoofnagle, Gerety, Smallwood et al.: "Subtyping of hepatitis B surface antigen and antibody by radioimmunoassay." in: Gastroenterology, Vol. 72, Issue 2, pp. 290-6, 1977 (PubMed). Tiollais, Pourcel, Dejean: "The hepatitis B virus." in: Nature, Vol. 317, Issue 6037, pp. 489-95, 1985 (PubMed). Beasley, Hwang, Lin et al.: "Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan." in: Lancet, Vol. 2, Issue 8256, pp. 1129-33, 1982 (PubMed). |
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Alternatives
Alternatives for antigen "Hepatitis B Surface Antigen (HBSAg)", type "Antibodies"
| Hosts | Mouse (75), Goat (20), Rabbit (12), Horse (4), Escherichia Coli (E. coli) (1) |
| Reactivities | Hepatitis B Virus (HBV) (49), Human (9) |
| Applications | ELISA (84), Immunoprecipitation (IP) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunofluorescence (IF) (11), Immunocytochemistry (ICC) (9), Immunoelectrophoresis (IEP) (8), Western Blotting (WB) (8), ELISA (Capture) (7), Lateral Flow (LF) (6), ELISA (Detection) (5), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), Affinity Purification (AP) (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1) |
| Conjugates | HRP (9), Biotin (4), FITC (4) |
| Epitopes | AA 132-137 (29), Subtype ad/ay (23), Subtype ayw (5), Subtype ay (2) |
