Raptor (Member of The TOR Signaling Pathway) (C-Term) antibody

Details for Product No. ABIN1003069
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Antigen
Epitope
C-Term
(1), (1)
Reactivity
Mouse (Murine)
(2), (2)
Host
Rabbit
(2)
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
(2), (2), (2)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN1003069
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Immunogen Raptor (CT) polyclol antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of mouse Raptor (Genbank accession No. Q8K4Q0).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif, mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.
Application Notes Raptor antibody can be used for the detection of Raptor by Western blot at 2 µg/ml. (Optimal dilution should be determined by user.) Raptor has multiple isoforms that may also be recognized by antibody. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hara, Yonezawa, Weng et al.: "Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14484-94, 1998 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Yonezawa, Tokunaga, Oshiro et al.: "Raptor, a binding partner of target of rapamycin." in: Biochemical and biophysical research communications, Vol. 313, Issue 2, pp. 437-41, 2003 (PubMed).

Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004 (PubMed).

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