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Receptor-Interacting serine-threonine Kinase 3 (RIPK3) antibody

Details for Product No. ABIN1003072, Supplier: Log in to see
Antigen
  • RIPK3
  • RIP3
  • 2610528K09Rik
  • AW107945
  • Rip3
  • Hcyp2
Reactivity
Mouse (Murine), Rat (Rattus)
115
39
15
7
6
4
2
1
Host
Rabbit
121
23
Clonality
Polyclonal
Conjugate
Un-conjugated
9
7
5
5
5
5
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
128
60
59
23
7
4
2
2
2
1
1
1
Supplier
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Immunogen Rabbit polyclol RIP3 antibody was raised against a peptide (AQFGRGRGWQPFHK) corresponding to amino acids 473 to 486 of murine RIP3 .
Blocking Peptide Blocking peptide for this product available: ABIN1004443
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Receptor Interacting Protein 3 (RIPK3 Antibody Abstract)
Background Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kappaB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
Pathways
Application Notes RIP3 antibody can be used for detection of RIP3 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) An approximately 57 kDa band can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Pazdernik, Donner, Goebl et al.: "Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6500-8, 2000 (PubMed).

Background publications Sun, Lee, Navas et al.: "RIP3, a novel apoptosis-inducing kinase." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 16871-5, 1999 (PubMed).

Yu, Huang, Shen et al.: "Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB." in: Current biology : CB, Vol. 9, Issue 10, pp. 539-42, 1999 (PubMed).