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DDX58 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58)

Details for Product anti-DDX58 Antibody No. ABIN1003083, Supplier: Log in to see
Antigen
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • RIG-I
  • RIGI
  • RLR-1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
157
78
25
21
1
Host
Rabbit
112
40
9
8
Clonality
Polyclonal
Conjugate
This DDX58 antibody is un-conjugated
12
7
6
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
122
51
35
33
23
12
10
3
3
3
2
2
2
1
Supplier
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Immunogen RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Retinoic Acid Inducible Gene 1 Protein (DDX58 Antibody Abstract)
Background The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.
Application Notes RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Akira, Uematsu, Takeuchi: "Pathogen recognition and innate immunity." in: Cell, Vol. 124, Issue 4, pp. 783-801, 2006 (PubMed).

Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, 2003 (PubMed).

Background publications Yoneyama, Kikuchi, Natsukawa, Shinobu, Imaizumi, Miyagishi, Taira, Akira, Fujita: "The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses." in: Nature immunology, Vol. 5, Issue 7, pp. 730-7, 2004 (PubMed).

Alexopoulou, Holt, Medzhitov, Flavell: "Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3." in: Nature, Vol. 413, Issue 6857, pp. 732-8, 2001 (PubMed).