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RIPK2 antibody (Receptor-Interacting Serine-threonine Kinase 2) (N-Term)

Details for Product anti-RIPK2 Antibody No. ABIN1003093, Supplier: Log in to see
Antigen
  • 2210420D18Rik
  • CARD3
  • CARDIAK
  • CCK
  • D4Bwg0615e
  • GIG30
  • RICK
  • RIP2
  • RIPK2
Alternatives
anti-Human RIPK2 antibody for Western Blotting
Epitope
N-Term
31
17
9
9
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
137
36
22
4
4
2
2
2
Host
Rabbit
117
16
4
Clonality
Polyclonal
Conjugate
This RIPK2 antibody is un-conjugated
6
5
4
2
2
2
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
124
69
41
17
15
12
3
3
3
3
1
Supplier
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Immunogen Rabbit polyclol RICK antibody was raised against a peptide corresponding to amino acids near the amino terminus of human RICK (Genbank accession no. O43353).
Blocking Peptide Blocking peptide for this product available: ABIN1004183
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name RIP-Like Interacting CLARP Kinase (RIPK2 Antibody Abstract)
Background Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kappaB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1beta. The messenger RNA of RICK is expressed in multiple human tissues.
Pathways TCR Signaling, Neurotrophin Signaling Pathway
Application Notes RICK antibody can be used for detection of RICK by Western blot at 0.5 - 1 µg/ml. (Optimal dilution should be determined by user.) A431 or K562 whole cell lysate can be used as positive control and an approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. RICK antibody is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Inohara, del Peso, Koseki, Chen, Núñez: "RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12296-300, 1998

McCarthy, Ni, Dixit: "RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 16968-75, 1998

Thome, Hofmann, Burns, Martinon, Bodmer, Mattmann, Tschopp: "Identification of CARDIAK, a RIP-like kinase that associates with caspase-1." in: Current biology : CB, Vol. 8, Issue 15, pp. 885-8, 1998