RPS6K1 antibody

Details for Product No. ABIN1003099, Supplier: Log in to see
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol RPS6K1 antibody was raised against a 16 amino acid peptide from near the amino-terminus of human RPS6K1 (Genbank accession No. AAH00479).
Blocking Peptide Blocking peptide for this product available: ABIN1004444
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name RPS6K1 (Kinase Involved in Cell Growth & Metabolism)
Background Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.
Application Notes RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 5 - 10 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Inoki, Ouyang, Li, Guan: "Signaling by target of rapamycin proteins in cell growth control." in: Microbiology and molecular biology reviews : MMBR, Vol. 69, Issue 1, pp. 79-100, 2005 (PubMed).

Manning: "Balancing Akt with S6K: implications for both metabolic diseases and tumorigenesis." in: The Journal of cell biology, Vol. 167, Issue 3, pp. 399-403, 2004 (PubMed).

Um, Frigerio, Watanabe, Picard, Joaquin, Sticker, Fumagalli, Allegrini, Kozma, Auwerx, Thomas: "Absence of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity." in: Nature, Vol. 431, Issue 7005, pp. 200-5, 2004 (PubMed).

Burnett, Barrow, Cohen, Snyder, Sabatini: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998 (PubMed).

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