Endoglin (ENG) antibody

Details for Product No. ABIN1003125, Supplier: Log in to see
Antigen
  • ENG
  • MGC137842
  • DKFZp469D0419
  • END
  • HHT1
  • ORW1
  • AI528660
  • AI662476
  • CD105
  • S-endoglin
  • endoglin
  • ENG
  • LOC100226246
  • Eng
Reactivity
Human
580
244
86
35
14
9
5
5
5
3
1
1
1
1
Host
Rabbit
395
225
124
5
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
66
63
50
21
20
17
13
13
12
11
11
9
9
9
9
8
8
8
7
6
6
5
4
4
4
3
3
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
487
458
187
151
117
98
95
94
81
16
15
13
12
8
7
7
6
6
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Options
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Immunogen Recombint human soluble CD105/Endoglin
Isotype IgG
Purification Protein A affinity chromatography (+ His tag depleted)
Alternative Name sCD105 (Endoglin) (ENG Antibody Abstract)
Background Produced from sera of rabbits immunized with highly pure recombinant human soluble CD105/Endoglin produced in insect cells. The recombinant soluble CD105/Endoglin consists of amino acid 26 (Glu) to 586 (Leu) and is fused to a C-terminal His-tag (6xHis).
Application Notes ELISA: Use at 1-15 µg/ml. Western Analysis: Use at a concentration of 1-2 µg/ml with the appropriate secondary reagents. FACS analysis and cell sorting: Use at 2-5 µg/ml together with the appropriate secondary reagents. Immunohistochemistry: Not investigated so far. Optimal dilutions should be determined by each laboratory for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from PBS (pH 7.4) w/o preservative
Preservative Without preservative
Handling Advice The lyophilized IgG is stable at 4 °C for at least six months and for greater than a year when kept at -20 °C.
Storage -20 °C
Product cited in: Parker, Goldring, Philip: "Endoglin is expressed on human chondrocytes and forms a heteromeric complex with betaglycan in a ligand and type II TGFbeta receptor independent manner." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 18, Issue 2, pp. 289-302, 2003 (PubMed).

Fonsatti, Del Vecchio, Altomonte, Sigalotti, Nicotra, Coral, Natali, Maio: "Endoglin: An accessory component of the TGF-beta-binding receptor-complex with diagnostic, prognostic, and bioimmunotherapeutic potential in human malignancies." in: Journal of cellular physiology, Vol. 188, Issue 1, pp. 1-7, 2001 (PubMed).

Arthur, Ure, Smith, Renforth, Wilson, Torsney, Charlton, Parums, Jowett, Marchuk, Burn, Diamond: "Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development." in: Developmental biology, Vol. 217, Issue 1, pp. 42-53, 2000 (PubMed).

Barbara, Wrana, Letarte: "Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 584-94, 1999 (PubMed).

McAllister, Grogg, Johnson, Gallione, Baldwin, Jackson, Helmbold, Markel, McKinnon, Murrell: "Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1." in: Nature genetics, Vol. 8, Issue 4, pp. 345-51, 1995 (PubMed).

Cheifetz, Bellón, Calés, Vera, Bernabeu, Massagué, Letarte: "Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19027-30, 1992 (PubMed).