STAT1 (JAK Activated Transcription) (C-Term) antibody

Details for Product No. ABIN1003193
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Antigen
Epitope
C-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN1003193
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Immunogen This antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1. The sequences differ from the murine corresponding sequences by four amino acids.
Isotype IgG
Purification Antibody is purified via DEAE-column chromatography.
Background STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.
Application Notes This antibody is human-reactive and can be used for Western blot at 1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 myg per sample. (Optimal dilution should be determined by user.) A 91 kDa band can be detected. No reaction to the 84 kDa STAT1alpha. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997 (PubMed).

Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998 (PubMed).

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