Stromal Interaction Molecule 1 (STIM1) antibody

Details for Product No. ABIN1003211
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Antigen
Synonyms stim1, MGC76045, STIM1, si:dkey-24p1.5, MGC154748, si:ch211-112l17.2, Stim1, D11S4896E, GOK, TAM, SIM
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(85), (46), (46), (25), (24), (18), (17), (2)
Host
Rabbit
(63), (22), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(70), (35), (24), (23), (14), (11), (10), (7), (1), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1003211
514.01 $
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Immunogen STIM1 antibody was raised in rabbits against a 24 amino acid peptide from near the carboxy terminus of human STIM1.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name STIM1
Background In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
Application Notes STIM1 antibody can be used for detection of STIM1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Zhang, Yu, Roos et al.: "STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane." in: Nature, Vol. 437, Issue 7060, pp. 902-5, 2005 (PubMed).

Spassova, Soboloff, He et al.: "STIM1 has a plasma membrane role in the activation of store-operated Ca(2+) channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 11, pp. 4040-5, 2006 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Luik, Lewis: "New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells." in: Trends in molecular medicine, Vol. 13, Issue 3, pp. 103-7, 2007 (PubMed).

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