Sumo (Small Ubiquitin-Like Modifier 1) antibody

Details for Product No. ABIN1003222, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Sumo antibody was raised against a 14 amino acid peptide from near the amino terminus of human sumo .
Blocking Peptide Blocking peptide for this product available: ABIN1004469
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist, Sumo antibody will only recognize isoform 1.
Application Notes Sumo antibody can be used for detection of sumo by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Gill: "Something about SUMO inhibits transcription." in: Current opinion in genetics & development, Vol. 15, Issue 5, pp. 536-41, 2005 (PubMed).

Dohmen: "SUMO protein modification." in: Biochimica et biophysica acta, Vol. 1695, Issue 1-3, pp. 113-31, 2004 (PubMed).

Matunis, Coutavas, Blobel: "A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex." in: The Journal of cell biology, Vol. 135, Issue 6 Pt 1, pp. 1457-70, 1997 (PubMed).

Background publications Desterro, Rodriguez, Hay: "SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation." in: Molecular cell, Vol. 2, Issue 2, pp. 233-9, 1998 (PubMed).