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TAR DNA Binding Protein (TARDBP) (Internal Region) antibody

Details for Product No. ABIN1003253, Supplier: Log in to see
Antigen
  • 1190002A23Rik
  • ALS10
  • C85084
  • DKFZp459I2127
  • TARDBP
  • tardbp
  • TDP-43
  • TDP43
  • Tdp43
  • wu:fb77f02
  • wu:fc52g10
Epitope
Internal Region
27
17
16
13
13
10
8
7
7
4
3
3
3
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
71
54
11
8
8
8
7
6
4
3
2
1
Host
Rabbit
111
33
15
1
Clonality
Polyclonal
Conjugate
Un-conjugated
6
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
140
70
51
39
28
15
11
10
5
5
4
3
2
Supplier
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Immunogen TDP43 antibody was raised in rabbits against an 18 amino acid peptide from near the center of human TDP43.
Blocking Peptide Blocking peptide for this product available: ABIN1003981
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name TDP43 (TARDBP Antibody Abstract)
Background TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3’ splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences. At least two isoforms are known to exist for this protein, this TDP43 antibody only recongizes the longer of the two isoforms.
Application Notes TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Neumann, Sampathu, Kwong, Truax, Micsenyi, Chou, Bruce, Schuck, Grossman, Clark, McCluskey, Miller, Masliah, Mackenzie, Feldman, Feiden, Kretzschmar, Trojanowski, Lee: "Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis." in: Science (New York, N.Y.), Vol. 314, Issue 5796, pp. 130-3, 2006

Product cited in: Buratti, Dörk, Zuccato, Pagani, Romano, Baralle: "Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping." in: The EMBO journal, Vol. 20, Issue 7, pp. 1774-84, 2001

Ou, Wu, Harrich, García-Martínez, Gaynor: "Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs." in: Journal of virology, Vol. 69, Issue 6, pp. 3584-96, 1995