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TMED10 antibody (Transmembrane Emp24-Like Trafficking Protein 10 (Yeast)) (C-Term)

Details for Product anti-TMED10 Antibody No. ABIN1003291, Supplier: Log in to see
Antigen
  • 1110014C03Rik
  • fe06g04
  • p23
  • P24(DELTA)
  • p24delta1
  • S31I125
  • S31III125
  • Tmp-21-I
  • Tmp21
  • TMP21
  • tmp21
  • wu:fb98e10
  • wu:fe06g04
  • zgc:85681
Epitope
C-Term
39
13
10
5
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
84
43
43
4
2
1
1
1
1
1
1
1
Host
Rabbit
71
14
2
Clonality
Polyclonal
Conjugate
This TMED10 antibody is un-conjugated
4
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
63
41
25
14
11
4
2
Supplier
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Immunogen TMP21 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TMP21.
Blocking Peptide Blocking peptide for this product available: ABIN1003898
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name TMP21 (TMED10 Antibody Abstract)
Background TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer’s disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.
Application Notes TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Chen, Hasegawa, Schmitt-Ulms, Kawarai, Bohm, Katayama, Gu, Sanjo, Glista, Rogaeva, Wakutani, Pardossi-Piquard, Ruan, Tandon, Checler, Marambaud, Hansen, Westaway, St George-Hyslop, Fraser: "TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity." in: Nature, Vol. 440, Issue 7088, pp. 1208-12, 2006

Blum, Feick, Puype, Vandekerckhove, Klengel, Nastainczyk, Schulz: "Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 17183-9, 1996

Product cited in: Periz, Fortini: "Functional reconstitution of gamma-secretase through coordinated expression of presenilin, nicastrin, Aph-1, and Pen-2." in: Journal of neuroscience research, Vol. 77, Issue 3, pp. 309-22, 2004

Selkoe: "The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease." in: Trends in cell biology, Vol. 8, Issue 11, pp. 447-53, 1999