Transmembrane Emp24-Like Trafficking Protein 10 (Yeast) (TMED10) (C-Term) antibody

Details for Product No. ABIN1003291
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Antigen
Synonyms tmp21, P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, p23, 1110014C03Rik, Tmp21, p24delta1, fe06g04, wu:fb98e10, wu:fe06g04, zgc:85681
Epitope
C-Term
(19), (6), (5), (5), (2), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(56), (42), (39), (15), (15), (13), (3), (2), (1), (1), (1)
Host
Rabbit
(56), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(51), (24), (20), (15), (10), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
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Catalog No. ABIN1003291
514.01 $
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Immunogen TMP21 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TMP21.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name TMP21
Background TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer’s disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.
Application Notes TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Blum, Feick, Puype et al.: "Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 17183-9, 1996 (PubMed).

Selkoe: "The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease." in: Trends in cell biology, Vol. 8, Issue 11, pp. 447-53, 1999 (PubMed).

Periz, Fortini: "Functional reconstitution of gamma-secretase through coordinated expression of presenilin, nicastrin, Aph-1, and Pen-2." in: Journal of neuroscience research, Vol. 77, Issue 3, pp. 309-22, 2004 (PubMed).

Chen, Hasegawa, Schmitt-Ulms et al.: "TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity." in: Nature, Vol. 440, Issue 7088, pp. 1208-12, 2006 (PubMed).

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