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ADAM17 antibody (ADAM Metallopeptidase Domain 17) (C-Term)

Details for Product anti-ADAM17 Antibody No. ABIN1003300, Supplier: Log in to see
Antigen
  • TACE
  • adam17
  • si:dkey-81b15.2
  • adam18
  • cd156b
  • csvp
  • tace
  • CD156b
  • Tace
  • ADAM18
  • CD156B
  • CSVP
  • NISBD
Alternatives
anti-Human ADAM17 antibody for Western Blotting
Epitope
C-Term
82
18
14
14
14
10
7
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
206
65
56
2
2
1
1
1
1
1
Host
Rabbit
171
25
16
1
1
Clonality
Polyclonal
Conjugate
This ADAM17 antibody is un-conjugated
9
8
6
6
5
5
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
173
117
27
26
21
13
12
8
7
5
3
2
1
1
Supplier
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Immunogen Rabbit polyclol TACE antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human TACE. This sequence differs from those of mouse and rat TACE by one amino acid.
Blocking Peptide Blocking peptide for this product available: ABIN1003882
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name TNF-alpha Converting Enzyme (ADAM17 Antibody Abstract)
Background Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
Pathways Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes TACE antibody can be used for detection of TACE by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) HeLa or Jurkat whole cell lysate can be used as positive control and 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. TACE antibody is human, mouse, and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Mizui, Yamazaki, Sagane et al.: "cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter." in: Gene, Vol. 233, Issue 1-2, pp. 67-74, 1999 (PubMed).

Peschon, Slack, Reddy et al.: "An essential role for ectodomain shedding in mammalian development." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1281-4, 1998 (PubMed).

Background publications Moss, Jin, Milla et al.: "Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. ..." in: Nature, Vol. 385, Issue 6618, pp. 733-6, 1997 (PubMed).

Black, Rauch, Kozlosky et al.: "A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells." in: Nature, Vol. 385, Issue 6618, pp. 729-33, 1997 (PubMed).