TLR9 antibody (Toll-Like Receptor 9) (Internal Region)

Details for Product anti-TLR9 Antibody No. ABIN1003343, Supplier: Log in to see
Antigen
  • CD289
  • toll-like receptor 9
  • TLR9
  • Tlr9
Alternatives
anti-Rat (Rattus) TLR9 antibody for Cytometry by Time of Flight
Epitope
Internal Region
40
33
16
13
12
11
8
8
7
6
6
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
261
166
57
39
25
22
8
6
4
2
2
2
2
2
1
1
1
Host
Rabbit
142
121
54
14
4
3
Clonality
Polyclonal
Conjugate
This TLR9 antibody is un-conjugated
28
27
19
6
6
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
245
179
106
103
90
40
37
36
15
7
6
5
5
3
3
3
3
3
2
1
Options
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Immunogen Rabbit polyclol TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9 (GenBank accession no. AAH32713).
Blocking Peptide Blocking peptide for this product available: ABIN1004049
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name Toll-Like Receptor 9 (TLR9 Antibody Abstract)
Background Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Application Notes TLR9 antibody can be used for detection of TLR9 by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Nishiya, DeFranco: "Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 19008-17, 2004 (PubMed).

Wagner: "The immunobiology of the TLR9 subfamily." in: Trends in immunology, Vol. 25, Issue 7, pp. 381-6, 2004 (PubMed).

Takeda, Kaisho, Akira: "Toll-like receptors." in: Annual review of immunology, Vol. 21, pp. 335-76, 2003 (PubMed).

Janeway, Medzhitov: "Innate immune recognition." in: Annual review of immunology, Vol. 20, pp. 197-216, 2002 (PubMed).