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RNF216 antibody (Ring Finger Protein 216)

Details for Product anti-RNF216 Antibody No. ABIN1003372, Supplier: Log in to see
Antigen
  • 2810055g22rik
  • 2810055G22Rik
  • AI647468
  • AU019462
  • C86502
  • CAHH
  • F830018F18Rik
  • MGC147026
  • RGD1565135
  • RNF216
  • TRIAD3
  • triad3
  • U7I1
  • u7i1
  • ubce7ip1
  • Ubce7ip1
  • UBCE7IP1
  • UIP83
  • ZIN
  • zin
Reactivity
Mouse (Murine)
54
43
15
1
1
1
1
1
1
Host
Rabbit
73
Clonality
Polyclonal
Conjugate
This RNF216 antibody is un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
56
26
22
20
12
4
4
3
2
Supplier
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Immunogen Rabbit polyclol TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A (GenBank accession no. AAP47174).
Blocking Peptide Blocking peptide for this product available: ABIN1004484
Isotype IgG
Purification Ion exchange chromatography purified.
Alternative Name TRIAD3A (TLR Ubiquitinating Enzyme) (RNF216 Antibody Abstract)
Background Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9, this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
Application Notes TRIAD3A antibody can be used for detection of TRIAD3A by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Takeda, Kaisho, Akira: "Toll-like receptors." in: Annual review of immunology, Vol. 21, pp. 335-76, 2003 (PubMed).

Wald, Qin, Zhao, Qian, Naramura, Tian, Towne, Sims, Stark, Li: "SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling." in: Nature immunology, Vol. 4, Issue 9, pp. 920-7, 2003 (PubMed).

Product cited in: Sweet, Leung, Kang, Sogaard, Schulz, Trajkovic, Campbell, Xu, Liew: "A novel pathway regulating lipopolysaccharide-induced shock by ST2/T1 via inhibition of Toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 11, pp. 6633-9, 2001 (PubMed).